An investigation of BRAF mutation in papillary thyroid carcinoma and its clinical value.
- Author:
Ri-xiang GONG
1
;
Yang ZHOU
;
Shu-hua LUO
;
Lin ZHANG
;
Bin ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma, Papillary; diagnosis; genetics; Adult; Aged; Carcinoma, Papillary; diagnosis; genetics; DNA Mutational Analysis; Female; Humans; Lymphatic Metastasis; genetics; Male; Middle Aged; Neoplasm Invasiveness; diagnosis; pathology; Neoplasm Recurrence, Local; diagnosis; pathology; Prognosis; Proto-Oncogene Proteins B-raf; genetics; Thyroid Neoplasms; diagnosis; genetics; Young Adult
- From: Chinese Journal of Medical Genetics 2009;26(3):310-313
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship between BRAF mutation and clinicopathological features in papillary thyroid carcinoma (PTC).
METHODSFresh samples were examined for the presence of BRAF mutations in 43 patients with PTC and 20 patients with non-PTC thyroid disease and 40 normal thyroid tissues by polymerase chain reaction (PCR) and direct sequencing. The relationship between BRAF mutation and clinicopathological features was studied.
RESULTSBRAF mutation was detected in 39.5% (17/43) of PTC samples, in 0 of non-PTC thyroid disease samples and normal thyroid tissues. Significant association was seen between BRAF mutation and both extrathyroidal invasion and cervical lymph node metastasis (P<0.05, P<0.05). There was no significant relationship between BRAF mutation and gender, age at the time of diagnosis, tumor size and distant metastasis.
CONCLUSIONBRAF mutation is associated with extrathyroidal invasion and lymph node metastasis. It may increase the ability of invasion and metastasis of PTC and have influence on prognosis.
