Effect of osteopontin silencing by lentivirus-mediated delivery of siRNA on glioma cell invasion and apoptosis.
- Author:
Wei YAN
1
;
Chun-fa QIAN
;
Lei SHI
;
Jin QIAN
;
Zhen FU
;
Ning LIU
;
Ai-lin LU
;
Yong-ping YOU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Gene Silencing; Gene Transfer Techniques; Genetic Vectors; genetics; metabolism; Glioma; genetics; metabolism; pathology; physiopathology; Humans; Lentivirus; genetics; metabolism; Neoplasm Invasiveness; Osteopontin; genetics; metabolism; RNA, Small Interfering; genetics
- From: Chinese Journal of Medical Genetics 2009;26(5):525-528
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of osteopontin silencing on the invasion and apoptosis of U251 cells.
METHODSThe invasion, apoptosis and levels of uPA, MMP-2 and MMP-9 were determined by invasion assay, flow cytometry, Western blot and real-time fluorescence quantitative PCR respectively.
RESULTSOsteopontin small interference RNA (siRNA) inhibited osteopontin expression and cell invasion, promoted apoptosis in U251 cells. In addition, the expression of Bcl-2, uPA, MMP-2 and MMP-9 was decreased, while Bax level was elevated.
CONCLUSIONOsteopontin siRNA can inhibit U251 cells invasion via the down-regulation of uPA, MMP-2 and MMP-9 levels, and promote apoptosis through induction of Bax expression and inhibition of Bcl 2 level. It suggests that osteopontin plays an important role in human glioma progression.
