Genetic polymorphisms of 9 non-combined of DNA index system short tandem repeat loci in Guangdong Han population.
- Author:
Jin-xiang ZHANG
1
;
Tian-yu XUE
;
Hai-xia LI
;
Hong-yu SUN
;
Jian-ding CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; ethnology; genetics; China; Genotype; Humans; Microsatellite Repeats; Polymorphism, Genetic
- From: Chinese Journal of Medical Genetics 2009;26(5):580-584
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the genetic polymorphisms and their forensic application of 9 non-combined of DNA index system (CODIS) short tandem repeat(STR) loci in Guangdong Han population.
METHODSDNA samples from 500 unrelated individuals were extracted and amplified with fluorescence labeled multiplex PCR system. PCR products were separated and genotyped with capillary electrophoresis.
RESULTSOne hundred and fifteen alleles and 160 genotypes were observed in the 9 STR loci, respectively. The heterozygosity was 0.824-0.884, the discrimination power (DP) was 0.925-0.969 and the polymorphism information content (PIC) was 0.77-0.86, respectively. The distribution met the Hardy-Weinberg equilibrium (P > 0.05). The total discrimination power was 1.00 x 10(-13), the combined probability of exclusion for trio-paternity testing was 0.999989488. The combined probability of exclusion for duo-paternity testing was 0.873436.
CONCLUSIONThe 9 STR loci are powerful and reliable for personal identification and paternity testing. They can be used as supplementary loci in fatherless (motherless) testing or cases with mutation events.
