Screening of differentially expressed genes from patients with Bardet-Biedl syndrome by gene chip analysis.
- Author:
Tao SHEN
1
;
Tao SHOU
;
Xinmin YAN
;
Hong DONG
;
Yunlian ZOU
;
Jianmei GAO
;
Rong ZENG
;
Yingkun HUA
;
Jinping ZHANG
;
Li LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Bardet-Biedl Syndrome; genetics; metabolism; Cells, Cultured; Female; Gene Expression Profiling; Gene Expression Regulation; Humans; Leukocytes, Mononuclear; metabolism; Male; Oligonucleotide Array Sequence Analysis; Siblings
- From: Chinese Journal of Medical Genetics 2009;26(6):648-652
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differential gene expression profile from patients with Bardet-Biedl syndrome (BBS) by oligonucleotide microarray technique.
METHODSTotal RNA of 3 probands with BBS and 4 healthy siblings were isolated from peripheral blood mononuclear cells and reverse-transcribed to cDNAs. Then the cDNAs were subjected for microarray screening with Affymetrix U133 Plus 2.0 array. Genechip scanner was applied to screen the hybridization signals. Genes differentially expressed between the BBS probands and controls were identified by using GCOS1.4 software with the standard of two-fold change (P<0.05) of expression.
RESULTSFifteen genes were up-regulated 2 or more fold and another 15 genes were down-regulated 2 or more fold in the BBS patients, among them 12 genes were related to signaling pathway and cell cycle by Gene Ontology (GO) analysis.
CONCLUSIONThe differentially expressed genes identified may correlate with the function or structure of cilia. Their roles in the BBS genesis need to be further studied.
