Studies on the seed embryo germination and propagation of Dendrobium candidum in vitro.
- Author:
Gui-xiang TANG
1
;
Fu-deng WANG
;
Wei-jun ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Benzyl Compounds; Carbon; pharmacology; Culture Media; Dendrobium; growth & development; Germination; drug effects; Kinetin; pharmacology; Naphthaleneacetic Acids; pharmacology; Plant Growth Regulators; pharmacology; Plants, Medicinal; growth & development; Purines; Seeds; growth & development; Tissue Culture Techniques; methods
- From: China Journal of Chinese Materia Medica 2005;30(20):1583-1586
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine optimum culture conditions for the seed embryo culture and rapid propagation of Dendrobium candidum.
METHODSeed embryos of D. candidum were incubated in the medium containing a combination of 6-benzylaminopurine (BA) and 1-naphthaleneacetic acid (NAA), potato extract, banana extract and activated carbon in order to induce seed embryo germination, protocorm differentiation, plantlet propagation and plantlet rooting.
RESULT AND CONCLUSIONThe maximum embryo germination percentage was obtained in the 1/2 MS media supplemented with 20% potato extract. The 1/2 MS medium supplemented with 1.0 mg x L(-1) BA and 0.1 mg x L(-1) NAA was very beneficial to the protocorm differentiation and propagation of D. candidum. The highest protocorm propagation index was obtained from the medium containing the activated carbon. The highest root numbers and length were observed in plants growing in 1/2 MS medium containing 0.5 mg x L(-1) NAA.
