Effect of Kanglaite injection oncyclooxygenase activity in lung carcinoma A549 cell.
- Author:
Qing-hua DONG
1
;
Xian ZHONG
;
Shu ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; administration & dosage; isolation & purification; pharmacology; Carcinoma, Non-Small-Cell Lung; enzymology; pathology; Cell Line, Tumor; Coix; chemistry; Cyclooxygenase 1; biosynthesis; genetics; Cyclooxygenase 2; biosynthesis; genetics; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Gene Expression Regulation, Neoplastic; Humans; Injections; Lung Neoplasms; enzymology; pathology; Plant Oils; administration & dosage; isolation & purification; pharmacology; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; Seeds; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(20):1621-1633
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Kanglaite injection on cyclooxygenase activity in lung carcinoma A549 cells.
METHODSThe expression of mRNA of COX-1 and COX-2 were measured by RT-PCR. The expression of COX-2 protein was measured by Western-blot.
RESULTKanglaite injection could selectively decreased COX-2 mRNA expression and protein expression while COX-1 mRNA expression was unchanged.
CONCLUSIONKanglaite injection is selectively inhibitory agent of COX-2, and possess inhibitory effects on cyclooxygenase 2.
