Determination of C21 steroidal glycosides in Marsdenia tenacissima by colorimetric method.
- Author:
Fan SHEN
1
;
Dao-feng CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Colorimetry; methods; Glycosides; analysis; Marsdenia; chemistry; Plant Stems; chemistry; Plants, Medicinal; chemistry; Powders; Quality Control; Reproducibility of Results; Steroids; analysis
- From: China Journal of Chinese Materia Medica 2005;30(22):1745-1748
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a quantitative method to deter mine C21 steroidal glycosides in Marsdenia tenacissima.
METHODMethanol was used as the extraction solvent and the samples were purified by macroporous resin ADS-7. A mixture of sulfuric acid-methanol (4:1)was used as color-producing reagent. The absorbance was measured at 325 nm.
RESULTThere is a good linearity (r = 0.999 9, n = 6) within the range of 10.6-148.4 microg. The average recoveries of tenacissoside-H at different concentrations were 95.8% to 97.1% with RSD less than 2.4% (n = 5).
CONCLUSIONThis method is reliable as a quantitative analytical method for the quality assessment of M. tenacissima.
