Determination of molecular weight and content of Ginkgo biloba exocarp polysaccharides by HPLC.
- Author:
Ping LU
1
;
Li REN
;
Ai-hua XU
;
Hua-sheng CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Fruit; chemistry; Ginkgo biloba; chemistry; Molecular Weight; Plants, Medicinal; chemistry; Polysaccharides; analysis; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2005;30(22):1749-1751
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the molecular weight and content of Ginkgo biloba exocarp polysaccharides.
METHODThe analysis was carried on a PL aquagel-OH MIXED (7.5 mm x 300 mm, 8 microm) chromatography column eluted with water as mobile phase at 1.0 mL x min(-1) of flow rate, the column temperature was 25 degrees C and the eluate was detected by RID.
RESULTThe average molecular weight of Ginkgo bilobaexocarp polysaccharides was 11 062.5 with RSD = 0.78% (n = 6); the content was 81.9% with RSD = 2.5% (n = 6), the standard curves of dextran (MW 12 000) were linear in the range of 1-20 microg, r = 0.999 9. The average recovery is 97.9%, RSD was 2.5%.
CONCLUSIONThis method was found to be sensitive and accurate for the measurement of Ginkgo biloba exocarp polysaccharides.
