Effect of different parts of Portulace oleracea on the levels of TNF-alpha and IL-6 in the supernatant of cultured adipose cell.
- Author:
Feng-ying XIAO
1
;
Fu-er LU
;
Li-jun XU
Author Information
- Publication Type:Journal Article
- MeSH: Adipocytes; cytology; metabolism; Animals; Cell Survival; drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Fatty Acids, Unsaturated; administration & dosage; isolation & purification; pharmacology; Flavones; administration & dosage; isolation & purification; pharmacology; Hyperlipidemias; blood; Interleukin-6; metabolism; Plants, Medicinal; chemistry; Portulaca; chemistry; Random Allocation; Rats; Rats, Wistar; Serum; physiology; Tumor Necrosis Factor-alpha; metabolism
- From: China Journal of Chinese Materia Medica 2005;30(22):1763-1766
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of drug-carried serum of the different parts of Portulace olerace on cytokine TNF-alpha and IL-6 secreted by adipose cell in vitro.
METHODModels of adipose cell were established by Rodbell method. Using the method of seropharmacology, the drug-carried serum of the different parts of P. olerace were prepared. The cell viability of each group was tested by. Methy thiazolyl tetrazolium (MTT) assay. The levels of TNF-alpha and IL-6 in the supernatant of cultured adipose cell were assayed by RIA.
RESULTMTT assay results showed the cell viability of normal serum group was significantly higher than that of high lipid serum( P < 0.05). Compared with the high lipid serum group, the cell viability of the drug-carried serum groups in 40% and 20% concentration were significantly increased( P < 0.05). The high lipid serum had a better effect on increasing the levels of TNF-alpha and IL-6 than the normal serum group (P < 0.01). Expect the drug-carried serum of P. olerace low dose group in 20% concentration, each drug-carried serum group could markedly lower the levels of TNF-alpha (P < 0.05 or P < 0.01). Each drug-carried serum group in 40% concentration and the drug-carried serum P. olerace MS high dose group in 20% concentration could markedly lower the levels of IL-6 (P < 0.05 or P < 0.01).
CONCLUSIONThe drug-carried serum of P. olerace and its different parts act on adipose cell damaged by the high lipid serum, significantly increas the cell viability in the groups in 40% and 20% concentration, and improve the disorder of lipid in differeut degrel by lowering the levels of TNF-alpha and IL-6 that adipose cell secreted in vitro.
