Role of mitochondria in Tubeimoside I-mediated apoptosis in human cervical carcinoma HeLa cell line.
- Author:
Fang WANG
1
;
Run-Di MA
;
Li-Jian YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cucurbitaceae; chemistry; Cyclosporine; pharmacology; Cytochromes c; metabolism; Dose-Response Relationship, Drug; HeLa Cells; Humans; Male; Membrane Potentials; drug effects; Mitochondria, Liver; physiology; secretion; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley; Saponins; administration & dosage; isolation & purification; pharmacology; Triterpenes; administration & dosage; isolation & purification; pharmacology
- From: China Journal of Chinese Materia Medica 2005;30(24):1935-1939
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of mitochondria in TBMS1-medited apoptosis of human cervical carcinoma HeLa cell line.
METHODMitochondrial transmembrane potentia (deltapsim) was assayed by flow cytometry. Apoptotic induction by TBMS1 was determined by gel electrophoresis of fragmented DNA. Cytochrome c (Cyt c) was detected by Western blotting.
RESULTThe results showed that TBMS1 induced apoptosis in HeLa cells, that TBMS1 decreased deltapsim and facilitated Cyt c release, and that TBMS1 induced apoptosis of HeLa cells dose-and time-dependently in accordance with increase of cytosolic Cyt c. TBMS1 had direct effect on isolated rat mitochondria which induced a time- and dose-dependent release of Cyt c from mitochondria. The results also showed that CsA protected partly HeLa cells from the effect of TBMS1.
CONCLUSIONThe mitochondrial apoptosis pathway has effects on TBMS1 induced human cervical carcinoma HeLa cell line apoptosis.
