Effect of Aloe coarse polysaccharide on cytokine secretion of keratinocytes in vitro.
- Author:
Xiao-Dong CHEN
1
;
Bo-Yu WU
;
Qiong JIANG
;
Li-Ying HUANG
;
Zhong-Cheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Aloe; chemistry; Cells, Cultured; Cytokines; secretion; Dose-Response Relationship, Drug; Epidermal Growth Factor; secretion; Humans; Interleukin-1beta; secretion; Interleukin-6; secretion; Interleukin-8; secretion; Keratinocytes; secretion; Plants, Medicinal; chemistry; Polysaccharides; administration & dosage; isolation & purification; pharmacology; Transforming Growth Factor alpha; secretion; Transforming Growth Factor beta1; secretion; Tumor Necrosis Factors; secretion
- From: China Journal of Chinese Materia Medica 2005;30(24):1944-1946
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of Aloe coarse polysaccharide on the levels of growth factors (EGF, TGF-alpha, TGF-beta1) and interleukins (IL-1beta, IL-6, IL-8) and tumor necrosis factor (TNF) in cultured keratinocytes.
METHODThe cultured keratinocytes were treated with Aloe coarse polysaccharide at concentrations of 75, 150, 300, 600, 1 200 mg x L(-1) land the equal volume of media as control group. The levels of EGF, TGF-alpha, TGF-beta1, IL-1beta, IL-6, IL-8 and TNF in the supernatants of cultured keratinocytes were assayed by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA).
RESULTCompared with the control group, the levels of EGF, TGF-alpha, IL-1beta, IL-6 and IL-8 were significantly increased by treatment with Aloe coarse polysaccharide (P < 0.05, P < 0.01) and in a dose dependent manner, and the levels of TGF-beta1 and TNF were also increased but no statistical significance.
CONCLUSIONAloe coarse polysaccharide may promote keratinocytes to secrete EGF, TGF-alpha, IL-1beta, IL-6 and IL-8.
