Experimental study of the effects of puerarin on biological characters of osteoblasts in vitro.
- Author:
Hong-Min ZANG
1
;
Jun-Chang CHEN
;
Yi-Heng LIU
;
Kun-Zheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Animals; Calcification, Physiologic; drug effects; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Isoflavones; pharmacology; Osteoblasts; cytology; enzymology; Rats; Rats, Sprague-Dawley; Skull; cytology
- From: China Journal of Chinese Materia Medica 2005;30(24):1947-1949
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of puerarin on cell proliferation, differentiation, maturation and mineralization in cultured rat osteoblasts.
METHODOsteoblasts from craniums of newly born SD rats were cultured in vitro. MTT, PNPP and ARS were used to observe the proliferation, activity of ALP and the number of mineral node of cultured osteoblasts in vitro.
RESULTIt was found that puerarin had the effect on stimulating cell proliferation, activity of ALP and the number of mineral node of cultured osteoblasts (P < 0.01 or P < 0.05).
CONCLUSIONPuerarin can promote proliferation, differentiation, maturation and mineralization of the osteoblasts in vitro.
