Taohong Siwu decoction II inhibits the angiogenesis and the expressions of VEGF and KDR/FLK-1 in C57BL/6J mice bearing B16 melanoma.
- Author:
Shu-mei WANG
1
;
Xiao-yu XU
;
Gang CHEN
;
Li-rong YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Female; Medicine, Chinese Traditional; Melanoma, Experimental; blood supply; metabolism; pathology; Mice; Mice, Inbred C57BL; Microcirculation; pathology; Neoplasm Transplantation; Neovascularization, Pathologic; pathology; Plants, Medicinal; chemistry; Random Allocation; Vascular Endothelial Growth Factor A; metabolism; Vascular Endothelial Growth Factor Receptor-2; metabolism
- From: China Journal of Chinese Materia Medica 2005;30(23):1866-1869
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Taohong Siwu decoction II on B16 melanoma in mice and the underlying mechanism.
METHODC57BL/6J mice bearing B16 melanoma were used in this study. The experimental groups were treated respectively with Taohong Siwu decoction II at doses of 2.5, 5 and 10 g x kg(-1) and cyclophosphamide at 0.05 g x kg(-1), Taohong Siwu decoction II at 10 g x kg(-1) plus cyclophosphamide at 0.025 mg x kg(-1). The tumor volume and weight, the expression of VEGF and KDR/FLK-1 and the amount of MVD were measured.
RESULTThe tumor volume and weight, the expression of VEGF and KDR/FLK-1 and the amount of MVD were reduced significantly after treatment with Taohong Siwu decoction II at the doses of 5 and 10 g x kg(-1) and Taohong Siwu decoction II combined with cyclophosphamide as compared with normal saline-treated group (P < 0.01).
CONCLUSIONTaohong Siwu decoction II can inhibit the growth of B16 melanoma in mice and attenuate the expressions of VEGF and KDR/FLK-1, suggesting that Taohong Siwu decoction II produces the antitumor effect via a possible antiagiogenisis mechanism.
