Construction and identification for cell strain of anti-human seminal plasma phospholipase A2 monoclonal antibody.
- Author:
Shu-Kui WANG
1
;
Yu-Feng HUANG
;
Xing-Yi XIA
;
Bao-Tong LI
;
Zi-Zheng WANG
;
Meng-Liu LIU
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Monoclonal; immunology; DEAE-Dextran; analogs & derivatives; chemistry; Electrophoresis, Polyacrylamide Gel; Humans; Molecular Weight; Phospholipases A; immunology; isolation & purification; metabolism; Phospholipases A2; Semen; enzymology
- From: National Journal of Andrology 2002;8(3):173-177
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo establish and evaluate the anti-human seminal plasma phospholipase A2 (PLA2) monoclonal antibody (McAb).
METHODSAfter having been separated and purified from human seminal plasma by PEG precipitation, Sephacryl S-300 column chromatography, DEAE-Sephadex A-25 column chromatography and HA column chromatography, PLA2 was regarded as an antigen to immune BALB/C mouse to produce anti-human seminal plasma PLA2 McAb. The PLA2 McAb sensitivity and specificity were performed by ELISA technique and Western-blot analysis, respectively.
RESULTSThe molecular weight of PLA2 depurated with 245 fold purification from human seminal plasma was about 34,900, while the sensitivity and typing of its McAb were 1:5(6)-1:5(8) and IgM (kappa) with a satisfied Western-Blot results.
CONCLUSIONSThe PLA2, which had not been reported in international and domestic papers, may be a new type of PLA2. The establish of its McAb will provide significant tools for the research of the relationship between PLA2 in human seminal plasma and male fertility.
