Effects of cyclosporin A on expression of FasL and Fas in the contralateral testis after the unilateral testis injured in KM mouse.
- Author:
Yong SHU
1
;
Guang-Yong YANG
;
Wei-Li ZHANG
;
Zi-Li HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cyclosporine; pharmacology; Disease Models, Animal; Fas Ligand Protein; Gene Expression; drug effects; Male; Membrane Glycoproteins; biosynthesis; Mice; Spermatogenesis; drug effects; Testis; drug effects; injuries; metabolism; pathology; fas Receptor; biosynthesis
- From: National Journal of Andrology 2002;8(3):201-206
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate the effects of Cyclosporin A (CsA) on spermatogenesis and expression of FasL and Fas in the contralateral testis after the unilateral testis was injured.
METHODS60 mice were randomly divided equally into groups A (control), B (the unilateral testis was injured by glacial acetic acid), C (excision of ipsilateral testis at 6 hours after the unilateral testis was injured by glacial acetic acid) and D (CsA within 6 hours after the unilateral testis was injured by glacial acetic acid). Sperm density and sperm motility were evaluated after 4 weeks. Expression of FasL and Fas was performed by immunohistochemistry (SP method). The positive cells with SP staining in seminiferous tubules were calculated.
RESULTSSperm density and sperm motility in group D were significantly increased compared with group B(P < 0.05). Expression of FasL and Fas in group D decreased significantly compared with group B (24.3 +/- 7.0 vs 37.8 +/- 5.8 and 17.8 +/- 4.3 vs 32.4 +/- 3.6, P < 0.05).
CONCLUSIONSCsA decreased expression of Fas and FasL and maintained spermatogenesis in the contralateral testis after the unilateral testis was injured by glacial acetic acid.
