The effects of antisenes oligodeoxynucleotide on the cyclic nucleotide monophosphates in smooth muscle cells of human corpus cavernosum.
- Author:
Wen-Jun BAI
1
;
Shu-Kun HOU
;
Xiao-Feng WANG
;
Zheng YAN
;
Pei-Ying HE
;
Qing-Ping DENG
;
Xiao-Peng HU
;
Kao-Peng GUAN
Author Information
- Publication Type:Journal Article
- MeSH: 3',5'-Cyclic-GMP Phosphodiesterases; antagonists & inhibitors; genetics; Cyclic AMP; metabolism; Cyclic GMP; metabolism; Cyclic Nucleotide Phosphodiesterases, Type 5; Humans; Male; Muscle, Smooth; drug effects; metabolism; Oligodeoxyribonucleotides, Antisense; pharmacology; Penis; cytology
- From: National Journal of Andrology 2002;8(2):88-91
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate the effects of antisense oligodeoxynucleotide(ASON) on the cyclic nucleotide monophosphates (cNMP) in smooth muscle cells of human corpus cavernosum, and provide experimental groundwork for the gene therapy of erectile dysfunction.
METHODSPDE5 gene ASON(containing exon 1) was transfected into the corpus cavernosum smooth muscle cells with the presence of liposome DOTAP. Another sense oligodeoxynucleotide(SON) and 1% of bovine serum were also transducted into the cells as controls. Two of cNMP, cAMP and cGMP, were probed and measured by ELISA at 1, 2, 4, 6, 10, 24 and 48 h after transfection.
RESULTSAfter transfection, the level of cGMP(1-6 h) in human corpus cavernosum smooth muscle cells was significantly higher than that in controls(P < 0.01).
CONCLUSIONSThe PDE5 gene ASON had been showed to manifest stimulative effect on the cGMP in smooth muscle cells of human corpus cavernosum in vitro, and it provides experimental groundwork for the gene therapy of erectile dysfunction.
