Effect of acrosin inhibitor KF-950 on acrosin activity and acrosome of human sperm.
- Author:
Ying ZHAN
1
;
Ya-Nan ZHAO
;
Zong-Lin LI
;
Li LI
;
Qian SONG
Author Information
- Publication Type:Journal Article
- MeSH: Acrosin; antagonists & inhibitors; Acrosome; metabolism; Humans; Male; Spermatozoa; drug effects; physiology
- From: National Journal of Andrology 2002;8(2):92-94
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo study the inhibitory effect of KF-950 on human acrosin and sperm acrosome.
METHODSHuman acrosin was extracted and purified with 2% acetic acid, and its residual activity was evaluated by BAEE/ADH assay after treated with different concentrations of KF-950. ABC assay was used to observe the effect of KF-950 on human acrosome with Biotin-PSA as a probe.
RESULTS1. The activity of normal sperm acrosin was (37.65 +/- 4.47) U/L. 2. The residual activity was inversely related to the concentration of KF-950 (r = -0.998), and had a dose-response curve. The result could be described by Y = 7.57-1.895X. 3. With increase of KF-950 concentration and prolongation of action time, the staining rate of acrosome obviously dropped (P < 0.01).
CONCLUSIONSKF-950 directly inhibits acrosin activity and assumely injures sperm acrosome. It might be a new kind of highly effective inhibitor.
