Preliminary study on hens immunized with recombinant human testis prostaglandin D synthase DNA.
- Author:
Jin-Chun LU
1
;
Yu-Feng HUANG
;
Xi-Ran ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies; analysis; Chickens; DNA, Recombinant; administration & dosage; immunology; Enzyme-Linked Immunosorbent Assay; Female; Genetic Vectors; Humans; Immunization; Intramolecular Oxidoreductases; genetics; immunology; Lipocalins; Male; Models, Animal; Plasmids; genetics; Testis; enzymology; Vaccines, DNA; administration & dosage; immunology
- From:National Journal of Andrology 2002;8(1):22-24
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESIn order to identify whether the hens immunized with recombinant human testis prostaglandin D synthase (rhtL-PGDS) DNA can produce anti-L-PGDS antibody.
METHODSThe serum were got from the hens immunized with recombinant plasmid pGEX-2T/htL-PGDS DNA (100 micrograms) every 2 weeks for 2 times. The exist of anti-L-PGDS antibody and its titer were tested with agarose dual immunodiffusion and ELISA with rhtL-PGDS as antigen.
RESULTSThe serum anti-L-PGDS antibody in hen immunized with pGEX-2T/htL-PGDS DNA were confirmed and its titer tested by ELISA was 1:2,048.
CONCLUSIONSIt is feasible to produce anti-L-PGDS antibody by immunizing hens with recombinant pGEX-2T/htL-PGES DNA.
