The effect of cryopreservation on human sperm chromosome.

Author: Jun PU ¹ ; Zai-Xian CHEN
Author Information

1. Department of Urology, First Hospital, Chongqing Medical University, Chongqing 400016, China.
Publication Type:Journal Article
MeSH: Chromosomes; Cryopreservation; Humans; Male; Spermatozoa; physiology
From: National Journal of Andrology 2002;8(1):45-47
CountryChina
Language:Chinese
Abstract:
OBJECTIVESTo evaluate the effects of cryopreservation on human sperm chromosome.
METHODSperm chromosome were acquired using in vitro fertilization of zona-free hamster oocytes and human sperm. The frequency of sperm chromosome anomalies and sex chromosomes ratio before and after freezing and with different freezing methods were compared.
RESULTSThere were no significant differences of frequency of sperm chromosome anomalies among fresh, fast frozen and slow frozen sperm (9.40%, 7.48% and 8.74%) (P > 0.75) or ratios of sex chromosomes (P > 0.90).
CONCLUSIONSThese studies indicate that cryopreservation does not exert effects on human sperm chromosome.