Extract of Zuojin Pill (characters: see text) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway.

Qiu-xian Peng; Hong-bing Cai; Jiang-li Peng; Kin Lam Yung; Jue Shi; Zhi-xian MO

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Extract of Zuojin Pill (characters: see text) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway.

Author: Qiu-xian PENG ¹ ; Hong-bing CAI ¹ ; Jiang-li PENG ² ; Kin Lam YUNG ³ ; Jue SHI ³ ; Zhi-xian MO ⁴
Author Information

1. School of Chinese Medicine, Southern Medical University, Guangzhou, 510515, China.
2. College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China.
3. Faculty of Science, Hong Kong Baptist University, Hong Kong SAR, China.
4. School of Chinese Medicine, Southern Medical University, Guangzhou, 510515, China. cherrymo@fimmu.com.
Publication Type:Journal Article
Keywords: Chinese medicine; Zuojin Pill; apoptosis; mitochondria-dependent pathway; reactive oxygen species
MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Blotting, Western; Cell Line, Tumor; Cell Survival; Colorimetry; Comet Assay; DNA Fragmentation; Drugs, Chinese Herbal; pharmacology; Flow Cytometry; Humans; Mitochondrial Membranes; drug effects; Reactive Oxygen Species; metabolism
From: Chinese journal of integrative medicine 2015;21(11):837-845
CountryChina
Language:English
Abstract:
OBJECTIVETo observe the effects of water extract of Zuojin Pill ([characters: see text], ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism.
METHODSEffects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9.
RESULTSZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation.
CONCLUSIONSZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.