Protective effects of Radix Astragali injection on multiple organs of rats with obstructive jaundice.

Zhe-wei Fei; Xi-ping Zhang; Jie Zhang; Xin-mei Huang; Di-jiong WU; Hong-hao BI

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Protective effects of Radix Astragali injection on multiple organs of rats with obstructive jaundice.

Author: Zhe-Wei FEI ¹ ; Xi-Ping ZHANG ² ; Jie ZHANG ³ ; Xin-Mei HUANG ³ ; Di-Jiong WU ³ ; Hong-Hao BI ⁴
Author Information

1. Department of General Surgery, Xinhua Hospital, Chongming Branch, School of Medicine, Shanghai Jiao Tong University, Shanghai, 202150, China.
2. Department of Breast Surgery, Zhejiang Cancer Hospital, Hangzhou, 310022, China. zxp99688@sina.com.
3. First College of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
4. Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, 50010, USA.
Publication Type:Journal Article
Keywords: Chinese medicine; Radix Astragali Injection; apoptosis; immunohistochemistry; multiple organ dysfunction; obstructive jaundice; tissue microarray
MeSH: Alanine Transaminase; blood; Animals; Apoptosis; drug effects; Aspartate Aminotransferases; blood; Bilirubin; blood; Blood Urea Nitrogen; Creatinine; blood; Drugs, Chinese Herbal; pharmacology; therapeutic use; Immunohistochemistry; Injections; Intercellular Adhesion Molecule-1; metabolism; Jaundice, Obstructive; blood; drug therapy; Kidney; drug effects; pathology; Liver; drug effects; pathology; Lung; drug effects; pathology; Male; NF-kappa B; metabolism; Organ Specificity; drug effects; Plant Extracts; pharmacology; Protective Agents; pharmacology; therapeutic use; Rats, Sprague-Dawley; Toll-Like Receptor 4; metabolism; bcl-2-Associated X Protein; metabolism; gamma-Glutamyltransferase; metabolism
From: Chinese journal of integrative medicine 2016;22(9):674-684
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the protective effects and mechanisms of Radix Astragali Injection on multiple organs of rats with obstructive jaundice (OJ).
METHODSA total of 180 rats were randomly divided into the sham-operated, model control and treated groups (60 in each group). On 7, 14, 21 and 28 days after operation, the serum contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), r-glutamyl transpeptidase (r-GT), total bilirubin (TBil), direct bilirubin (DBil), blood urine nitrogen (BUN), and creatinine (CREA) were determined. And the pathological changes of livers, kidneys and lungs, and protein expressions of toll-like receptor-4 (TLR-4) of livers, intercellular adhesion molecule-1 (ICAM-1) of lungs, Bax and nuclear factor-kappa B (NF-κB), as well as apoptotic indexes of multiple organs were observed, respectively.
RESULTSThe pathological severity scores of multiple organs (including livers on 7, 14, 21 and 28 days, kidneys on 14 and 28 days, and lungs on 14 days), serum contents of ALT (14 and 21 days), AST (14 days), TBil (7, 14, 21 and 28 days), DBil (14 and 21 days), BUN (28 days), protein expressions of TLR-4 (in livers, 28 days), Bax (in livers and kidneys, 21 days), and apoptotic indexes in livers (7 and 21 days) in the treated group were significantly lower than those in the model control group (P<0.05 or P<0.01).
CONCLUSIONRadix Astragali Injection exerts protective effects on multiple organs of OJ rats by improving the pathological changes of lung, liver and kidney, decreasing the serum index of hepatic and renal function as well as inhibiting the protein expression of TLR-4 and Bax in the livers and Bax in the kidneys.