Protective effect of salidroside on contrast-induced nephropathy in comparison with N-acetylcysteine and its underlying mechanism.
- Author:
Yue XING
1
;
Ri-bao WEI
;
Lu TANG
;
Yue YANG
;
Xiao-yong ZHENG
;
Zi-cheng WANG
;
Yu-wei GAO
Author Information
- Publication Type:Journal Article
- MeSH: Acetylcysteine; pharmacology; Animals; Contrast Media; adverse effects; Cytoprotection; drug effects; Glucosides; pharmacology; Kidney; drug effects; pathology; Kidney Diseases; chemically induced; prevention & control; Oxidative Stress; drug effects; Phenols; pharmacology; Rats; Rats, Wistar; Signal Transduction; drug effects
- From: Chinese journal of integrative medicine 2015;21(4):266-273
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the prevention effect of salidroside on contrast-induced-nephropathy (CIN) and its underlying mechanism.
METHODSA total of 24 Wistar rats were randomly divided into 4 groups with 6 in each group. Rats were firstly administrated with normal saline (control and model groups), N-acetylcysteine (NAC, NAC group) and salidroside (salidroside group) for 7 days before model establishment in each group, respectively. Histopathological analysis was performed by periodic acid-Schiff (PAS) staining. Oxidative stress related parameters including superoxide dismutase (SOD) and methane dicarboxylic aldehyde (MDA), nitric oxide (NO), angiotensin II (Ang II), 8-hydroxy-2'-deoxyguanosine (8-OHdG), mRNA and protein levels of endothelial nitric oxide synthase (eNOS), and nitric oxide synthase (NOS) activity were measured.
RESULTSCompared with the control group, the levels of MDA, Ang II and 8-OHdG were all significantly increased and levels of SOD, NO, and eNOS mRNA and protein were decreased significantly in the model group (P<0.05). Meanwhile, the NOS activity was also significantly decreased in the model group (P<0.05). In addition, the levels of these parameters were all improved in the NAC (P<0.05) and salidroside groups and no significant different was found between these two groups (P>0.05).
CONCLUSIONSalidroside can be the potential substitute of NAC to prevent CIN. The underlying mechanism may be associated with oxidative stress damage caused by contrast agents.
