Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes.
- Author:
Wei-Wei WEN
1
;
Xin SUN
2
;
Hai-Feng ZHUANG
3
;
Xiao-Jie LIN
3
;
Zhi-Yin ZHENG
3
;
Rui-Lan GAO
3
;
Li-Ming YIN
4
Author Information
- Publication Type:Journal Article
- Keywords: differentiation; gene expression profile; megakaryocyte; panaxadiol saponins; proliferation
- MeSH: Blotting, Western; Bone Marrow Cells; cytology; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Colony-Forming Units Assay; Drugs, Chinese Herbal; pharmacology; Flow Cytometry; Gene Expression Profiling; Ginsenosides; pharmacology; Humans; Megakaryocytes; cytology; drug effects; metabolism; Patents as Topic; Saponins; pharmacology; Stem Cells; cytology; drug effects; Transcription Factors; metabolism; Up-Regulation; drug effects; genetics
- From: Chinese journal of integrative medicine 2016;22(1):28-35
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of panaxadiol saponins component (PDS-C) isolated from total saponins of panax ginseng on proliferation, differentiation and corresponding gene expression profile of megakaryocytes.
METHODSBone marrow culture of colony forming assay of megakaryocytic progenitor cells (CFU-MK) was observed for the promoting proliferation mediated by PDS-C, and differentiation of megakaryocytic blasts caused by PDS-C was analyzed with flow cytometry in CHRF-288 and Meg-01 cells, as well as proliferation, differentiation-related genes expression profile and protein expression levels were detected by human gene expression microarray and western blot.
RESULTSIn response to PDS-C 10, 20 and 50 mg/L, CFU-MK from 10 human bone marrow samples was increased by 28.9%±2.7%, 41.0%±3.2% and 40.5%±2.6% over untreated control, respectively (P <0.01, each). Flow cytometry analysis showed that PDS-C treated CHRF-288 cells and Meg-01 cells significantly increased in CD42b, CD41, TSP and CD36 positive ratio, respectively. PDS-C induced 29 genes up-regulated more than two-fold commonly in both cells detected by human expression microarray representing 4000 known genes. The protein expression levels of ZNF91, c-Fos, BTF3a, GATA-1, RGS2, NDRG2 and RUNX1 were increased with western blot in correspond to microarray results.
CONCLUSIONPDS-C as an effective component for hematopoiesis, play the role to enhance proliferation and differentiation of megakaryocytes, also up-regulated expression of proliferation, differentiation-related genes and proteins in vitro.
