Culture of the Human Glomerular Endothelial Cells.
- Author:
Su Kil PARK
1
;
Han Jong AHN
;
Seong Who KIM
;
Jae Dam LEE
;
Jung Sik PARK
Author Information
1. Department of Internal Medicine, College of Medicine, University of Ulsan, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Cell culture;
Human glomerular endothelial cells;
Endothelial cell growth factor(ECGF)
- MeSH:
Actins;
Cell Culture Techniques;
Collagenases;
Endothelial Cells*;
Epithelial Cells;
Fibronectins;
Heparin;
Humans*;
Keratins;
Population Characteristics
- From:Korean Journal of Nephrology
1997;16(2):221-229
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Whereas mesangial and epithelial cells from glomeruli are commonly grown in vitro, there has been major difficulties in developing homogenous cultures of human glomerular endothelial cells. This study defines the conditions for the reproducible isolation and growth of homogenous monolayers of human glomerular endothelial cells based on the method of Green DF et al published in 1992. Using the selective media and the sieving method, fibronectin was required as a surface matrix after adequate collagenase treatment, and endothelial cell growth factor and heparin was needed for the continuous growth of endothelial cells. The endothelial cell growth factor was isolated from the bovine hypothalamic extracts. Glomerular capillary endothelial cells exhibited a cobblestone morphology at confluence and stained homogenously with von Willebrand factor(factor VIII). The cytokeratin and the actin were not stained. This study might be helpful for in vitro study to know the biological characteristics of human glomerular endothelial cells under the predetermined condition.
