Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α.
- Author:
Shuai YAN
1
;
Lan YANG
2
;
Yin-Zi YUE
1
;
Wen-Lin LI
2
;
Li ZENG
3
;
Jin YUE
4
;
Chun-Qin MAO
5
Author Information
- Publication Type:Journal Article
- Keywords: experimental research; ligustrazine; peritoneal mesothelial cells; transforming growth factor β/Smad
- MeSH: Animals; Collagen Type I; genetics; metabolism; Epithelium; drug effects; metabolism; Fibronectins; metabolism; Male; Nanoparticles; chemistry; ultrastructure; Particle Size; Peritoneal Cavity; cytology; Pyrazines; pharmacology; RNA, Messenger; genetics; metabolism; Rats, Sprague-Dawley; Signal Transduction; drug effects; Smad Proteins; metabolism; Transforming Growth Factor beta; genetics; metabolism; Tumor Necrosis Factor-alpha; pharmacology
- From: Chinese journal of integrative medicine 2016;22(8):629-634
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the effect of ligustrazine nanoparticles nano spray (LNNS) on transforming growth factor β (TGF-β)/Smad signal protein of rat peritoneal mesothelial cells (RPMC) induced by tumor necrosis factor α (TNF-α), and the anti-adhesion mechanism of LNNS in the abdominal cavity.
METHODSThe primary culture and subculture of rat peritoneal mesothelial cells (RPMC) was processed by trypsin digestion method in vitro. The third generation was identifified for experiment and divided into 5 groups: a blank group: RPMC without treatment; a control group: RPMC stimulated with TNF-α; RPMC treated by a low-dosage LNNS group (2.5 mg/L); RPMC treated by a medium-dosage LNNS group (5 mg/L); and RPMC treated by a high-dosage LNNS group (10 mg/L). Reverse transcription-polymerase chain reaction was applied to test the expression of fifibronectin, collagen I (COL-I), TGF-β mRNA, and Western blot method to test the Smad protein 7 expression of RPMC.
RESULTSCompared with the blank group, a signifificant elevation in fifibronectin (FN), COL-I and TGF-β mRNA expression of RPMC were observed in the control group (P<0.05). Compared with the control group, LNNS suppressed the expressions of FN, COL-I and TGF-β mRNA in a concentrationdependent manner (P<0.05). The expression of Smad7 protein of RPMC was down-regulated by TNF-α stimulation, and up-regulated with the increase of LNNS dose (P<0.05).
CONCLUSIONSTNF-α may induce changes in RPMC's viability, leading to peritoneal injury. LNNS could reverse the induction of fifibrosis related cytokine FN, COL-I and TGF-β, up-regulating the expression of Smad7 by TNF-α in RPMC, thus attenuate peritoneal injury by repairing mesothelial cells.
