Whole-cell recording of potassium and sodium currents in cortical, hippocampal, and sympathetic ganglia neurons.
- Author:
Wen-yu CUI
1
;
Yin-li ZHANG
;
Xiao-feng YIN
;
Hai WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium Channels; physiology; Cells, Cultured; Cerebral Cortex; physiology; Ganglia, Sympathetic; cytology; physiology; Hippocampus; physiology; Membrane Potentials; physiology; Patch-Clamp Techniques; Potassium Channels; physiology; Rats; Rats, Wistar; Sodium Channels; physiology
- From: Chinese Journal of Applied Physiology 2005;21(1):105-110
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo establish the culturing of cortical, hippocampal and sympathetic ganglia neurons derived from neonatal rats and the whole-cell recording techniques of potassium and sodium currents in cultured neurons.
METHODSCortex, hippocampus and sympathetic ganglion were isolated from neonatal rats (1-3 days) and digested by trypsin (0.125%). The neurons were cultured in polylysine covered petri dish (O35 mm) with DEME medium(high glucose) for seven days for electrophysiological experiments.
RESULTSThe neurons cultured by this method were in good conditions and could be sealed easily in whole-cell recording. I(Na), I(A), and I(K) could be recorded respectively in cultured neurons by different recording paradigms in Ca2+ -free extracellular solution.
CONCLUSIONIt is a practicable and economical method for electrophysiological study of nervous system.
