Distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes.

Author: Hong FENG ¹ ; Wen-lin XIU ; Rui ZHAN ; Ling-jia QIAN
Author Information

1. Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
Publication Type:Journal Article
MeSH: Animals; Animals, Newborn; Cells, Cultured; Green Fluorescent Proteins; genetics; metabolism; Myocytes, Cardiac; metabolism; Plasmids; Rats; Rats, Wistar; Transfection
From:Chinese Journal of Applied Physiology 2005;21(1):112-114
CountryChina
Language:Chinese
Abstract:
AIMTo study the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes.
METHODSThe neonatal rat cardiomyocytes were cultured. According to the different grow period of neonatal rat cardiomyocytes, the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes were studied.
RESULTSThe efficiency was significantly increased after pEGFP-N1 plasmid transfection in one-day cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.
CONCLUSIONThe efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes was related to the grow period of neonatal rat cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.