AIMTo culture adrenal cortical cells of rat in primary monolayer form.

METHODSCollagenase and hyaluronidase incubation were used to isolate adrenocortical cells of male Wistar rats. The isolated adrenocortical cells were incubated in the DMEM solution supplied with 15% NBS, at the temperature of 37 degrees C, and the air contained 5% CO2. Identification was done by 3bet-HSD dyeing.

RESULTSThe cells which had the relatively big cell body and a few projections were dyed. There was a phenomenon of coexistence of epithelial-like cells and fibroblastic cells.

CONCLUSIONRats monolayer adrenocortical cell culture was successful.