Effect of adenosine on three dimensional tube formation and angiogenesis of human umbilical vein endothelial cell (HUVEC) in vitro.
- Author:
Yan WANG
1
;
Jian-hua SHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine; pharmacology; Cells, Cultured; Cellular Structures; drug effects; Human Umbilical Vein Endothelial Cells; cytology; drug effects; Humans
- From:Chinese Journal of Applied Physiology 2005;21(2):160-162
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the influence of adenosine on three-dimensional HUVEC tube formation.
METHODSA new three-dimensional culture type was established in which collagen type I was the substance and two layers of HUVEC grew upward and downward separately. In control group no adenosine was added in the holes; in experiment group 10(-4) mol/L adenosine was added in each hole. Under inverted phase contrast microscope, defined sights were observed and the number of sprout was recorded. Observation lasted for 96 hours, and experiments were repeated 3 times.
RESULTSHUVEC grew upward and downward into collage gel respectively. Tube structure and three-dimensional network was built up gradually. In experiment group(adenosine 10(-4) mol/L) HUVEC grew fast with quick infiltration and sprouting. The tube formation was thick and might form three-dimensional network throughout the collagen gel. At each time point, the difference between experiment and control group was significant (P < 0.05 or P < 0.01).
CONCLUSIONAdenosine may promote HUVEC sprouting and tube-formation.
