Construction of antisense recombinant adenoviral vector for c-myc and its antiproliferative effect on rat lymphocytes.
- Author:
Ying-ge LIU
1
;
Hao-wen QI
;
Huan-zhang LI
;
Ming-quan SU
;
Wen-bin YU
;
Yue-yun MA
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Antisense Elements (Genetics); Cell Line; Cell Proliferation; Genes, myc; genetics; Genetic Vectors; Lymphocytes; cytology; Rats; Thymus Gland; cytology
- From:Chinese Journal of Applied Physiology 2005;21(2):220-223
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the antiproliferative effect of antisense recombinant adenoviral vector for c-myc on rat thymus lymphocytes.
METHODSAntisense and sense bacterial plasmids for c-myc were constructed. Bacterial plasmids and El detected adenoviral plasmid were cotransfected into 293 cells. Recombinant adenoviral vectors were obtained after cotransfection. The antiproliferative effects were assayed by MTS. The expression of c-myc mRNA was detected by RT-PCR.
RESULTSThe results showed that antisense recombinant adenoviral vector for c-myc could inhibit rat thymus lymphocytes proliferation. The expression of c-myc mRNA was decreased after antisense recombinant adenoviral vector for c-myc was transfected into cells.
CONCLUSIONRecombinant antisense adenoviral vector for c-myc could inhibit rat thymus lymphocytes proliferation.
