The changes of cAMP and adenylyl cyclase mRNA level in hippocampus of mice with ischemia/reperfusion.
- Author:
Wei-Bin WANG
1
;
Chun-Feng SONG
;
Pei-Yuan LU
;
Yu YIN
;
Cui-Ping LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenylyl Cyclases; genetics; metabolism; Animals; Cyclic AMP; genetics; metabolism; Hippocampus; metabolism; Male; Mice; Mice, Inbred Strains; RNA, Messenger; genetics; Reperfusion Injury; genetics; metabolism
- From: Chinese Journal of Applied Physiology 2005;21(3):278-280
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the cAMP and adenylyl cyclase (AC) mRNA level in hippocampus of mice with vascular dementia (ischemia/reperfusion), and explore the molecular pathogenesis of ischemia/reperfusion.
METHODSThe mice were subjected for ischemia/reperfusion three times on bilateral common carotid arteries by knots to establish models of ischemia/reperfusion and the changes of learning and memory were tested on 29 d/30 d after operation. Sham-operation mice were introduced as control group. The cAMP level was evaluated by the radioimmunoassay (RIA), AC mRNA positive neurons of hippocampus CA1 area were dyed through in-situ hybridization (ISH).
RESULTSCompared with sham-operation group, the learning and memory of model group was worse (P < 0.05), the cAMP level in hippocampus was lower (P < 0.05) and the surface density (Sv) of AC mRNA positive neurons reduced (P < 0.05).
CONCLUSIONThe lower cAMP and AC mRNA level in hippocampus might participate in the molecular pathogenesis of ischemia/reperfusion.
