Effects of hypoxia on the secretions of proinflammatory cytokines TNF-alpha and IL-6 in macrophages and its mechanism.
- Author:
Cui-Ping ZHANG
1
;
Yin-Zhi XIE
;
Peng CHEN
;
Xin HONG
;
Hong-Jing NIE
;
Hong-Bin DONG
;
Zhao-Yun YIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Hypoxia; Cells, Cultured; Interleukin-6; metabolism; Macrophages; secretion; Male; Mice; Mice, Inbred Strains; NF-kappa B; metabolism; Oxygen; metabolism; Tumor Necrosis Factor-alpha; metabolism
- From:Chinese Journal of Applied Physiology 2005;21(3):281-284
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of hypoxia on the secretions of proinflammatory cytokines TNF-alpha and IL-6 and to inquire into the mechanism.
METHODSSeparated mice abdominal macrophages which were identified with non-specific esterase dye method, and created the hypoxic cultured model. The levels of TNF-alpha and IL-6 in the medium were determined by ELISA method. The mRNA expressions of TNF-alpha and IL-6 were measured by RT-PCR method. NF-kappaB activation was assayed by Western blot method. Finaly, we added cortone (5 microg/ml) to the medium, then observed the secretion levels of TNF-alpha and IL-6 during hypoxia.
RESULTSThe secretions of TNF-alpha and IL-6 from Mphi exposed to hypoxia for 12 h were increased significantly compared with control (P < 0.01). The expressions of TNF-alpha mRNA and IL-6 mRNA were enhanced obviously contrasted with control (P < 0.01). NF-kappaB activation in Mphi nuclei was raised at 2 h during hypoxia and persisted to 5 h. We added cortone to the medium and found no significant change in secretion of TNF-alpha and IL-6 during hypoxia.
CONCLUSIONHypoxia could activate NF-kappaB and make it shift to nucleus which promoted the transcriptions and expressions of TNF-alpha and IL-6.
