Protective effects of local heat preconditioning on hepatic ischemia/reperfusion injury.
- Author:
Li-Juan QIN
1
;
Yu CAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aspartate Aminotransferases; blood; HSP70 Heat-Shock Proteins; metabolism; Heat-Shock Response; Ischemic Preconditioning; Liver; blood supply; metabolism; Male; Rats; Rats, Wistar; Reperfusion Injury; prevention & control; Superoxide Dismutase; metabolism
- From: Chinese Journal of Applied Physiology 2005;21(3):285-288
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the protective mechanism of HSP70 induced by the heat stress pretreatment on the hepatic ischemia/ reperfusion (I/R) injury.
METHODSTo establish the models of the hepatic ischemia/ reperfusion injury using pringle's maneuver with or without heat stress pretreatment. The rats were randomly divided into pretreatment group (HP + I/R) and non-pretreatment group (I/R), in which the expression of HSP70, the MDA contents and SOD activity in liver, the activities of serum AST and ALT, and the pathological changes in liver were detected at 0, 4, 8, 12 and 24 h after I/R.
RESULTSAt any time point set after I/R, the SOD activity in the liver in HP + I/R group were higher than those in IR group, HSP70 expression maximum was attained at 12 h after heat pre-treatment. While in HP+ I/R group the levels of liver enzymes and the production of MDA significantly reduced and the pathological changes improved compared with those in I/R group.
CONCLUSIONHSP70 induced by heat pretreated protecting liver against I/R injury may be through increasing the SOD content and then reducing the insults of oxygen free radicals in the liver.
