Effects of vagotomy on UCP2 mRNA expression and gastric acid secretion in rats.
- Author:
Yuan ZOU
1
;
Mei YANG
;
De-Zheng GONG
;
Li-Li GUAN
;
Nan TIAN
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine Triphosphate; metabolism; Animals; Gastric Acid; secretion; Ion Channels; genetics; metabolism; Male; Mitochondrial Proteins; genetics; metabolism; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley; Uncoupling Protein 2; Vagotomy; Vagus Nerve; metabolism
- From: Chinese Journal of Applied Physiology 2005;21(3):290-292
- CountryChina
- Language:Chinese
-
Abstract:
AIMThe relationship between gastric acid secretion and ATP level, and regulation of uncoupling protein 2 (UCP2) mRNA expression by vagus nerve were studied in vagotomies rats.
METHODSWith the high selective vagotomy model, the gastric acidity was titrated to pH 7.0 with 0.1 mol/L NaOH solution and ATP contents were quantified by using fluorimetry. The expression of UCP2 mRNA was observed by using Northern blot in stomach of rats.
RESULTSBoth of gastric acidity and ATP contents in stomach body decreased significantly at 24 h after vagotomy. The expression of UCP2 mRNA was markedly increased as compared with sham operation group.
CONCLUSIONATP contents decreased and vagus nerve down-regulates expression of UCP2 mRNA in stomach corpus in vagotomies rats. The results indicates that vagus nerve could underlay the gastric acidity by inhibiting expression of UCP2 mRNA and increasing ATP contents in rats.
