Study on screening differentially expressed genes in mice livers by silver staining DD-PCR.
- Author:
Xin-Hong LUAN
1
;
Zhong-Ming HU
;
Wei-Quan LIU
;
Yu JIANG
;
Kai WANG
;
Yong-Kui WU
;
Qian-Xue LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Fatigue; metabolism; Gene Expression Profiling; methods; Liver; metabolism; Male; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Polymerase Chain Reaction; methods; RNA, Messenger; genetics; Silver Staining; Swimming
- From:Chinese Journal of Applied Physiology 2005;21(3):296-299
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo screen swimming-fatigue related genes in mice and lay theoretic basis for researching the molecular mechanism of fatigue.
METHODS30 male BALB/c mice (20 +/- 2g) were divided into control group, dipping in water group and swimming-fatigue group respectively. After fatigue for swimming in swimming-fatigue group, with control group and dipping in water group, liver tissues in mice were collected. With improved silver staining mRNA differential display method, the differentially expressed genes in mice livers were screened and evaluated by reversed Northern blot. The positive segments were analyzed homology by BLAST.
RESULTS7 of DD-ESTs were gained. Two of them only expressed in swimming-fatigue group, two down-regulated expressed, and three up-regulated. One of them was a novel gene and was accepted by GenBank, AY615302.
CONCLUSIONSeven DD-ESTs in swimming-fatigue mice were gained by silver staining mRNA differential display method.
