Effect of antisense oligonucleotides of ryanodine receptor on proliferation and Ca2+i of airway smooth muscle cells.
- Author:
Ying WANG
1
;
Sui-Yang ZHANG
;
Gui-Sheng QIAN
;
Xi-Liang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Calcium Channels; Cell Division; Cell Proliferation; drug effects; Cells, Cultured; Myocytes, Smooth Muscle; cytology; drug effects; metabolism; Oligonucleotides, Antisense; pharmacology; Rats; Respiratory System; Ryanodine Receptor Calcium Release Channel; genetics; pharmacology
- From: Chinese Journal of Applied Physiology 2005;21(3):324-328
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of antisense oligonucleotides (ASON) of ryanodine receptor on proliferation and [Ca2+]i concentration of airway smooth muscle cells (ASMCs).
METHODSASMCs were cultivated with collagen enzyme digestion method. Different concentrations of ASON were added to the cultures with Lipofectamine 2000 to observe the ASMCs proliferation using MTS/PES method. The changes of ASMCs [Ca2+]i were also observed by flow cytometry. The expression of mRNA of subtypes of RyR was assayed by RT-PCR.
RESULTSRyR ASON restrained the proliferation of ASMCs, decreased the expression of RyR and reduced the concentration of [Ca2+]i.
CONCLUSIONRyR ASON could inhibit the proliferation of ASMCs by influencing the concentration of [Ca2+]i after excited.
