Determination of homocysteine level in plasma by high performance liquids chromatography with fluorescence detection.
- Author:
Xu DA
1
;
Ling-Jia QIAN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Homocysteine; blood; Humans; Plasma; chemistry; Spectrometry, Fluorescence
- From: Chinese Journal of Applied Physiology 2005;21(3):353-355
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo establish a method for detection of plasma total homocysteine with HPLC.
METHODSThe chromatography analysis was carried out using a Symmetry Shield RP18. The mobile phase was sodium acetate (0.08 mol/L) and methanol (1%) and we utilized a HPLC system with fluorescence detection of plasma homocysteine derivatized from reaction with 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F).
RESULTSThe average recoveries were 95.8 - 100.8% and the relative standard deviations were 1.2-2.0%.
CONCLUSIONThe results showed it to be a rapid and accurate method for the determination of homocysteine level in plasma.
