A cell model of ischemia/reperfusion injury on skeletal muscle.
- Author:
Hong-Jie LI
1
;
Guang-Ling ZHANG
;
Lian-Yuan ZHANG
;
Shu-Yun DONG
;
Xiu-Li MEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Colorimetry; L-Lactate Dehydrogenase; analysis; Muscle, Skeletal; blood supply; Rats; Reperfusion Injury
- From: Chinese Journal of Applied Physiology 2005;21(3):356-358
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo establish a model of ischemia/reperfusion injury on L-6TG cell.
METHODSCultured L-6TG cells were divided into 2 groups: control group (C), ischemia/reperfusion group (I/R), LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochondria respiration were evaluated in each group, the micromorphologic changes were observed with microscope.
RESULTSCompared with control group, after L-6TG cell suffered ischemia 4 hours and reperfusion 4 hours, LDH in culture fluid, XOD, free calcium in L-6TG cell all increased significantly, while SOD in L-6TG cell and mitochondrial respiration decreased, structural damage to L-6TG cell was severe.
CONCLUSIONUsing mimicking ischemic solution and mimicking reperfusion solution can successfully establish a model of ischemia/reperfusion injury on L-6TG cell.
