Effect of spleen tyrosine kinase on the proliferation of pulmonary vascular smooth muscle cells in rats.
- Author:
Qin LUO
1
;
Zheng-Xiang GAO
;
Li-Li CAO
;
Li YU
;
Tao WANG
;
Han-Min LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Cells, Cultured; Hypertension, Pulmonary; pathology; Intracellular Signaling Peptides and Proteins; analysis; genetics; physiology; Male; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; cytology; Platelet-Derived Growth Factor; pharmacology; Protein-Tyrosine Kinases; analysis; genetics; physiology; Proto-Oncogene Proteins c-sis; Pulmonary Artery; cytology; Rats; Rats, Sprague-Dawley; Stilbenes; pharmacology; Syk Kinase
- From: Chinese Journal of Contemporary Pediatrics 2010;12(11):886-890
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of spleen tyrosine kinase (Syk) in rat pulmonary vascular smooth muscle cells (PVSMCs) proliferation induced by platelet-derived growth factor-BB (PDGF-BB).
METHODSPVSMCs from male Sprague-Dawley rats were cultured in vitro and the cells of passages 3-5 were used in the experiment. PVSMCs were stimulated by PDGF-BB and were treated with three different doses of piceatannol, a Syk selective inhibitor. Cell proliferation was assessed by methyl thiazolyl tetrazolium (MTT) assay. DNA synthesis was measured by ³H-thymidine incorporation (³H-TdR). Cellular cycle was observed by flow cytometry. Syk mRNA and protein expression were detected using real-time quantitative PCR and Western blot, respectively.
RESULTSThe expression of Syk protein of PVSMCs was significantly up-regulated following PDGF-BB stimulation. PDGF-BB stimulation dramatically increased PVSMCs proliferation. After piceatannol treatment, both Syk mRNA and protein expression decreased and the proliferation of PVSMCs was inhibited in a dose-dependent manner.
CONCLUSIONSSyk may promote PVSMCs proliferation induced by PDGF-BB.
