Expression of imprinted genes during the course of differentiation from mouse embryonic stem cells to islet-like cells in vitro.
- Author:
Feng LIU
1
;
Jia ZHEN
;
Min-Xiang LEI
;
Hui-Ling CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; drug effects; Insulin; Islets of Langerhans; cytology; Mice; Mouse Embryonic Stem Cells; Proteins; Reverse Transcriptase Polymerase Chain Reaction; Stem Cells
- From: Chinese Journal of Contemporary Pediatrics 2010;12(12):954-958
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of in vitro inducement on the expression of SF1-G imprinted genes, Kcnq1 and Cdkn1c during the course of differentiation from mouse embryonic stem (ES) cells to islet-like cells.
METHODSMouse ES cells were induced to differentiate into islet-like cells in vitro. The expression of islet specific markers was tested by RT-PCR or immunofluorescence. RT-PCR/RFLP was used to test the imprinted genes parental expression in cells at different stages.
RESULTSIslet specific genes, such as Insulin, Glucagon, Somatostatin, IAPP and Glut2, were expressed in differentiated cells. The proteins of insulin, C-peptide and Somastatin were expressed in the final stage cells. Imprinted gene Kcnq1 and Cdkn1c were biallelicly expressed in islet-like cells.
CONCLUSIONSMouse ES cells can be successfully induced into islet-like cells in vitro. Gene imprinting status of Kcnq1 and Cdkn1c may be changed in differentiated cells (causing loss of imprinting) during the in vitro inducement.
