Effects of TGF-β1 on gene expression of connective tissue growth factor in lung fibroblasts.
- Author:
Jian-Hua FU
1
;
Hai-Ping YANG
;
Li PAN
;
Xin-Dong XUE
;
Hong GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Connective Tissue Growth Factor; genetics; Female; Fibroblasts; metabolism; Gene Expression; drug effects; Lung; cytology; metabolism; Pulmonary Fibrosis; etiology; RNA, Messenger; analysis; Rats; Rats, Wistar; Transforming Growth Factor beta1; pharmacology
- From: Chinese Journal of Contemporary Pediatrics 2011;13(1):36-39
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of transforming growth factor-β1 (TGF-β1) on the gene expression of connective tissue growth factor (CTGF) in cultured lung fibroblasts of embryonic rats in vitro.
METHODSWistar rats of embryonic 19 days were used for primary culture of lung fibroblasts (LFs). The cells in the experimental group were treated by different concentrations (1, 5 or 10 ng/mL) and different durations (12, 24 or 48 hrs) of TGF-β1 to stimulate the LFs. The cells in the control group were cultured in serum-free medium. RT-PCR method was applied to detect CTGF mRNA expression in LFs.
RESULTSCompared with the control group, the levels of CTGF mRNA in LFs in the experimental group increased significantly (P<0.05). CTGF mRNA expression gradually increased with increasing concentration and duration of TGF-β1 treatment (P<0.05).
CONCLUSIONSTGF-β1 can stimulate CTGF gene expression in LFs and increase CTGF gene expression in a dose-and time-dependent manner.
