Effects of recombinant human erythropoietin on serum levels of neuron-specific enolase, S-100β protein and myelin basic protein in rats following status epilepticus.
- Author:
Chun-Ming JIANG
1
;
Jian-Min DU
;
Zhan-Li LIU
;
Li-Qiong CHEN
;
Mei FENG
;
Yi-Hua YANG
;
Jie-Hua WANG
;
Xian-Mei HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Erythropoietin; pharmacology; therapeutic use; Male; Myelin Basic Protein; blood; Nerve Growth Factors; blood; Phosphopyruvate Hydratase; blood; Rats; Rats, Sprague-Dawley; Recombinant Proteins; S100 Calcium Binding Protein beta Subunit; S100 Proteins; blood; Status Epilepticus; blood; drug therapy
- From: Chinese Journal of Contemporary Pediatrics 2011;13(1):50-52
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study examined the effect of recombinant human erythropoietin (r-HuEPO) on the serum levels of neuron-specific enolase (NSE), S-100β protein and myelin basic protein (MBP) in young rats 24 hrs after lithium-pilocarpine-induced status epilepticus (SE) in order to study the potential role of r-HuEPO in epileptic brain damage.
METHODSForty 19-21-day-old male Sprague-Dawley (SD) rats were randomly divided into four groups (n=10): normal control group, SE, r-HuEPO pretreated-SE and r-HuEPO. SE was induced by lithium-pilocarpine. R-HuEPO (500 IU/kg) was intraperitoneally injected in the r-HuEPO pretreated-SE and r-HuEPO groups 4 hrs before SE. Serum levels of NSE, S-100β and MBP were determined 24 hrs after the SE event.
RESULTSSerum levels of NSE, S-100β and MBP in the SE group increased significantly compared with those in the normal control and the r-HuEPO groups (P＜0.05). The r-HuEPO pretreated-SE group showed significantly decreased serum levels of NSE, S-100β and MBP compared with the SE group (P＜0.05).
CONCLUSIONSr-HuEPO may reduce the expression of NSE, S-100β and MBP and thus might provide an early protective effect against epileptic brain injury.
