Diallyl disulfide inhibits invasion and metastasis of MCF-7 breast cancer cells in vitro by down-regulating p38 activity.
- Author:
Xiao-Xiao CHEN
1
;
Xiao-Wang LIU
;
Zhi-Gang ZHOU
;
Xue-Yan CHEN
;
Li-Ding LI
;
Ting XIONG
;
Lu PENG
;
Jian TU
Author Information
- Publication Type:Journal Article
- MeSH: Allyl Compounds; pharmacology; Breast Neoplasms; pathology; Cadherins; metabolism; Disulfides; pharmacology; Gene Expression Regulation, Neoplastic; Humans; MAP Kinase Signaling System; drug effects; MCF-7 Cells; drug effects; Matrix Metalloproteinase 9; metabolism; Mitogen-Activated Protein Kinase 11; metabolism; Neoplasm Invasiveness; Neoplasm Metastasis; Transforming Growth Factor beta1; pharmacology; Vimentin; metabolism
- From: Journal of Southern Medical University 2016;36(6):814-818
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of diallyl disulfide (DADS) on invasion and metastasis of human breast cancer MCF-7 cells and explore the possible mechanism.
METHODSMCF-7 cells treated with 100, 200, and 400 µmol/L of DADS for 24 h were examined for cell invasion and migration capacities using Transwell assay and wound healing assay, respectively. The protein expression of E-cadherin, vimentin, MMP-9 and p-p38 in the cells were detected with Western blotting. The effect of transforming growth factor-β1 (TGF-β1) as the agonist of p38 activity was tested in antagonizing the effects of DADS.
RESULTSDADS inhibited the invasion and migration of MCF-7 cells in a dose-dependent manner, down-regulated the protein expression of Vimentin and MMP-9 and up-regulated E-cadherin expression in the cells. Treatment with TGF-β1 to up-regulate p38 activity obviously antagonized the inhibitory effect of DADS on the invasion and metastasis of MCF-7 cells.
CONCLUSIONDADS can inhibit the invasion and metastasis of MCF-7 cells in vitro by down-regulating p38 activity.
