Docosahexaenoic acid inhibits aflatoxin B1-induced migration and invasion in hepatocellular carcinoma cells in vitro.
- Author:
Ke-He CHEN
1
;
Ting GAO
;
Jin-Fei PAN
;
Hai-Ming WEI
;
Chun-Hong JIA
;
Jiao LAN
;
Zhen-Xiang CHEN
;
Deng PAN
;
Xiao-Chun BAI
Author Information
- Publication Type:Journal Article
- MeSH: Aflatoxin B1; pharmacology; Carcinoma, Hepatocellular; pathology; Cell Cycle; Cell Movement; drug effects; Docosahexaenoic Acids; pharmacology; Golgi Apparatus; Hep G2 Cells; Humans; Liver Neoplasms; pathology; Mitochondria; Neoplasm Invasiveness
- From: Journal of Southern Medical University 2016;36(7):952-956
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of docosahexaenoic acid (DHA) on invasiveness of aflatoxin B1 (AFB1)-induced hepatocellular carcinoma cells in vitro.
METHODSHepG2.2.15 cells were exposed to different concentrations of AFB1 and DHA plus AFB1. The cell migration and invasion were assessed using wound-healing and Transwell assay, and flow cytometry was used to analyze the cell cycle changes. The ultrastructural changes of the cells were observed by transmission electron microscopy.
RESULTSCompared with the control group, the cells exposed to2 µmol/L AFB1 showed obviously enhanced migration and invasion with decreased cell ratio in G1/G1 phase and increased cell ratio in G2/M phase but no changes in S phase cells; transmission electron microscopy revealed the presence of multiple nucleoli and significantly increased mitochondria and Golgi apparatus in the exposed cells. Compared with AFB1-exposed cells, the cells treated with DHA and AFB1 showed decreased migration and invasion abilities, and the G1/G1 phase cells increased and G2/M phase cells decreased significantly; ultrastructurally, the cells contained single nucleoli with decreased mitochondria and vacuolization occurred in the cytoplasm.
CONCLUSIONDHA can significantly inhibit AFB1-induced enhancement of cell migration and invasion in hepatocellular carcinoma cells in vitro.
