Effect of parathyroid hormone (1-34) on expression of matrix Gla protein and Wnt/β catenin signaling pathways in MG63 cell lines.
- Author:
Ya-Li HU
1
;
Jie ZHANG
;
Liu-Chen FU
;
Ya YANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Calcium-Binding Proteins; metabolism; Cell Differentiation; Cell Line, Tumor; Cell Survival; Extracellular Matrix Proteins; metabolism; Humans; Intercellular Signaling Peptides and Proteins; pharmacology; Osteogenesis; Osteoporosis; Parathyroid Hormone; pharmacology; Real-Time Polymerase Chain Reaction; Wnt Signaling Pathway
- From: Journal of Southern Medical University 2016;36(7):984-989
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of parathyroid hormone (PTH)(1-34) on the expression of matrix Gla protein (MGP) and Wnt/β-catenin signaling pathway and elucidate the possible molecular mechanism of PTH (1-34) in the prevention and treatment of osteoporosis.
METHODSMG63 cells treated with PTH (1-34) at 10(-9), 10(-8), and 10(-7) mol/L, alone or in combination with Wnt/β-catenin signaling pathway inhibitors DKK-1 (200 ng/ml) were examined for mRNA and protein expressions related with Wnt/β-catenin signaling with real-time PCR and Western blotting. The cell differentiation after the treatment was assessed with alkaline phosphatase (ALP) staining and cell viability assay.
RESULTSPTH (1-34) significantly increased the expression of MGP in a dose-dependent manner in MG63 cells (P<0.05 or P<0.01). PTH treatment obviously enhanced ALP activity in the cells, and this effect was suppressed by DKK-1. Combined treatment with DKK-1 partially blocked PTH-induced enhancement of ALP activity (P<0.05). PTH promoted the expression of MGP and enhanced LRP5, β-catenin, and Runx2 expressions in Wnt/β-catenin signaling pathway at both protein and mRNA levels (P<0.05 or P<0.01). DKK-1 partially blocked the effect of PTH (1-34) on Wnt/β-catenin signaling pathway (P<0.05) without affecting MGP expression.
CONCLUSIONPTH (1-34) significantly increases the expressions of MGP and proteins in the Wnt/β-catenin signaling pathway. Wnt/β-catenin signaling pathway and MGP mediate the regulation of osteogenosis by PTH.
