Effect of miR-101 on biological characteristics of colorectal cancer cell line SW620.
- Author:
Yan LIU
1
;
Yan-Xia LU
;
Min ZHOU
;
Lin ZHENG
;
Xue-Nong LI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Cycle Checkpoints; Cell Line, Tumor; Cell Proliferation; Colorectal Neoplasms; genetics; Humans; MicroRNAs; genetics; Neoplasm Invasiveness
- From: Journal of Southern Medical University 2016;36(7):990-996
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of miR-101 on biological behaviors of colorectal cancer cell line SW620.
METHODSCCK-8 method, colony formation assay, cell cycle analysis and apoptosis analysis were applied to assess the effects of miR-101 on cell proliferation, invasion and apoptosis of SW620 cells.
RESULTSOver-expression of miR-101 in SW620 cells significantly suppressed the cell proliferation and attenuated the colony-forming ability of the cells. Flow cytometry showed that over-expression of miR-101 in SW620 cells caused obvious cell cycle arrest in G2/M and 1/ phases, and significantly increased the cell apoptosis rate.
CONCLUSIONOver-expression of miR-101 can inhibit the proliferation, cause cell cycle arrest and promote apoptosis of colorectal cancer SW620 cells.
