Rapid establishment of suspension cell lines in japonica rice.
- Author:
Hongyu ZHANG
1
;
Fuyun YANG
;
Mei GAO
;
Peizhou XU
;
Quanfang ZHANG
;
Jiandi XU
;
Xianjun WU
Author Information
1. Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, China.
- Publication Type:Journal Article
- MeSH:
Cell Culture Techniques;
Cell Differentiation;
physiology;
Cell Line;
Culture Media;
Oryza;
cytology
- From:
Chinese Journal of Biotechnology
2009;25(3):424-427
- CountryChina
- Language:Chinese
-
Abstract:
With Jingkang No.5 (PiA), calli of the PiA induced for 10-15 days were transferred into amino acid liquid culture medium, to establish excellent rice suspension cell lines successfully in a relative short time. The growth characteristics and differentiation conditions of suspension cells were measured at different phases. Results revealed that the optimal subculture time was 7-10 days, and the cells cultured for 30-120 days had the best differentiation ability (57.1%) and regeneration ability (20%). This study is promising in further using the suspension cell for genetic transformation and protoplasm isolation.
