Analysis of PARP10 tissue expression profile, interactive protein and UV stress reaction.
- Author:
Mengbin YU
1
;
Lixia ZHAO
;
Yutao YANG
;
Zhixin YANG
;
Yingying ZHANG
;
Hengqi ZHU
;
Xiaowei ZHOU
;
Peitang HUANG
Author Information
1. Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
- Publication Type:Journal Article
- MeSH:
Actins;
metabolism;
Animals;
Humans;
Mice;
Poly(ADP-ribose) Polymerases;
metabolism;
radiation effects;
Protein Interaction Domains and Motifs;
Proto-Oncogene Proteins;
metabolism;
radiation effects;
Stress, Physiological;
radiation effects;
Tissue Distribution;
Ultraviolet Rays
- From:
Chinese Journal of Biotechnology
2009;25(3):428-434
- CountryChina
- Language:Chinese
-
Abstract:
One pair of primers were designed and synthesized based on the cDNA sequence encoding Homo sapiens poly (ADP-ribose) polymerase family, member 10 (PARP10) reported on the GenBank. The cDNA sequence encoding PARP10 was cloned from 293FT cell by RT-PCR. Then the RT-PCR product was cloned into pCMV-Myc and pEGFP-C1 plasmids. The interaction between PARP10 and beta-actin was identified through immuno-precipitation and laser confocal microscopy. Extensive expression of PARP10 in mouse tissues was confirmed by RT-PCR. Besides, Western blotting analysis indicated that cell injury caused by UV treatment could promote the expression of PARP10. The results in this paper would benefit further study of PARP10.
