Establishment of a GFP-based cellular model for screening novel proteasome inhibitors.
- Author:
Haitong FANG
1
;
Zheng HU
;
Guangbiao ZHOU
Author Information
1. Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China.
- Publication Type:Journal Article
- MeSH:
Boronic Acids;
pharmacology;
Bortezomib;
Cell Line;
Drug Evaluation, Preclinical;
Drugs, Chinese Herbal;
isolation & purification;
pharmacology;
Genetic Vectors;
genetics;
Green Fluorescent Proteins;
biosynthesis;
genetics;
Humans;
Lentivirus;
genetics;
metabolism;
Models, Biological;
Protease Inhibitors;
isolation & purification;
pharmacology;
Pyrazines;
pharmacology
- From:
Chinese Journal of Biotechnology
2009;25(3):452-456
- CountryChina
- Language:Chinese
-
Abstract:
To establish a green fluorescent protein (GFP)-based cellular model for screening proteasome inhibitors from compounds including extracts from Traditional Chinese Medicinal herbs, we transfected A549 cells with lentivirus expression vector pGC-E1-ZU1-GFP, and selected clones stably expressing ZU1-GFP. The A549-ZU1-GFP cells were treated with PS-341 for 24 h, and the accumulation of GFP was analyzed by fluorescence microscope. We found that the fluorescence intensity of A549-ZU1-GFP cells treated with PS-341 was significantly increased as compared to the control. We screened for proteasome inhibitors from compounds including some from Traditional Chinese Medicinal herbs, and the data suggested a few compounds could be novel proteasome inhibitors.
