Optimization of high-cell-density fermentation process for S-adenosyl-L-methionine production.
- Author:
Jiepeng WANG
1
;
Jinjun HAN
;
Xiaonan LI
;
Peiyi LIU
;
Tianwei TAN
Author Information
1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
- Publication Type:Journal Article
- MeSH:
Adenosine Triphosphate;
pharmacology;
Fermentation;
Phosphates;
pharmacology;
S-Adenosylmethionine;
biosynthesis;
genetics;
Saccharomyces cerevisiae;
enzymology;
genetics;
Sodium Glutamate;
pharmacology
- From:
Chinese Journal of Biotechnology
2009;25(4):533-536
- CountryChina
- Language:Chinese
-
Abstract:
Poor stability existed in the anaphase of the high-cell-density fermentation of Saccharomyces crevisiae for S-adenosyl-L-methionine (SAM) production in 5 L fermentor. To improve the fermentation stability, we studied the addition of diammonium hydrogen phosphate, sodium glutamate and adenosine disodium triphosphate into glucose feeding solution. Study of four fed-batch cultures showed that, after 34 h fermentation, when dry cell weight reached 100 g/L, the addition of 50 g pre-L-methionine and glucose feeding with 10 g/L adenosine disodium triphosphate was optimal for SAM production. Under this condition, after 65.7 h fermentation, both the dry cell weight and the yield of SAM reached the maximum, 180 g/L and 17.1 g/L respectively.
